BIOTECHNOLOGY AND BIOCHEMISTRY

Aiming at development of molecular markers and molecular maps in fodder crops, understanding and utilizing apomixis in perennial grasses, biochemical basis f stress tolerance, and generation of interspecific hybrids.

Identification of obligate sexual lines in C. ciliaris and guinea grass, and molecular markers for mode of reproduction.

Identification of molecular markers (SSR) in perennial grasses, berseem, oats and Stylosanthes.

Multigene control model for regulation of apomixis and Identification of reproductive pathways of seed development in apomictic grasses.

Generation of worlds' largest ploidy series in a crop plant utilizing Hybridization-supplemented Apomixis-components Partitioning Approach (HAPA) from a single progenitor (2n=4x=32) in guinea grass, represented by 3x, 4x, 5x, 6x, 7x, 8x, 9x and 11x cytotypes. ¢ Identification of differentially expressed genes during apomeosis in Pennisetum.

Tissue culture: Established protocol and micropropagation in Berseem, Oats, C. ciliaris; Embryo rescue in Berseem, Pearl millet; In vitro screening (salinity): Berseem, Oats

Cytogenetics: Identification/generation of Chromosomal races in Pearl millet (2x, 3x, 4x) (MS/MF/Maintainers), Berseem (2x, 4x); P. pedicellatum (4x, 6x, 8x), P. squamulatum (8x), P. orientale (4x, 6x); and Oats (aneuploids) (6x+)

Transformation in Lucerne, C. ciliaris, Dichanthium

Identitifcation of differentially expressed genes during apomeiosis in Pennisetum