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ICAR-Indian Grassland and Fodder Research InstituteICAR-Indian Grassland and Fodder Research Institute
(Indian Council of Agricultural Research)
Near Pahuj Dam, Gwalior Road, Jhansi - 284 003 (UP) India
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  0510-2730666, 2730158
  FAX: 0510-2730833
Instrument Details
Instrument/Facility Name: Lyophilizer
Model: Scanvac cool safe
Lyophilization is a process whereby a product is dried by removing the water under low temperature and pressure. Lyophilization involves the removal of water or other solvents from a given product by a process called sublimation. This occurs when the ice of a frozen product converts directly to the gaseous state without passing through the liquid phase. This enables the preparation of a stable product that is easy to use and store at ambient temperatures.A low pressure environment is pre-requisite to allow this process to take place. In order to start the removal of water, the pressure inside the freeze dryer must be below the “tripple point value” for the product, whilst also maintaining the temperature of the sample below its freeze point in the lyophilization process. Pre-freezing – first stage of the lyophilization processThe sample is frozen, which means the water in the product is converted to ice, thereby the phase has changed from liquid to solid. Slow pre-freezing will produce lager ice crystals, which are easier to lyophilize, whilst fast pre-freezing results in smaller crystals.Primary drying – Second stage of the lyophilization process- In the second stage of lyophilization the sublimation process starts. The ice formed in the pre-freeze step is removed from the sample by the direct transition of the “solid” ice to a vapour without passing through a liquid phase. The resultant vapour is collected by the condenser, which has a lower temperature and pressure than the product. The vapour is thus converted back to ice on the condenser surface.The “energy” required for this process to occur is provided by a gentle heating of the sample, which willstart the sublimation process and eventually the sample will dry.If too much energy (heat) is applied to the sample during this stage the condenser of the lyophilizer may not be able to condense the volume of vapours fast enough, the ice condenser temperature will subsequently rise along with its vapor pressure, thus increasing the risk of the sample melting.Secondary drying – Third stage of the lyophilization process- Finally, any residual water present, which is strongly bound to the molecules of the sample, is converted tovapour and removed from the sample.This water has invariably a vapour pressure lower than that of water in its “free” form.Removal of the water in this final stage of lyophilization will be performed at higher product temperatures, consequently, any biological activity of the sample will not be impaired or affected. This usually involves increasing the temperature and lowering the pressure to provide enough energy to break down the molecular bonding. A process called desorption. CoolSafe is an outstanding and versatile range of advanced bench-top freeze dryers. It offers -Lowest condenser temperatures of -55°C, -95°C, -100°C or -110°C, Seamless stainless steel condenser with external cooling coils, heavily insulated to conserve energy and increase performance, Easy draining with built-in valve, allowing removal of water after a completed freeze-drying session, Easy operation with a built-in vacuum valve connecting the condenser and vacuum pump and allowing the condenser to cool down and the vacuum pump to warm up separately, ensuring an efficient start-up sequence.
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Working Principles:
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Lyophilized/freeze dried products retain their original properties. By adding water or other solvents they can be reconstituted easily and very quickly to a usable solution. There is a wide range of substances for which freeze drying/Lyophilization is used as conservation/preservation method without changing their composition.
User Instructions:
Notes during operation During test, cares shall be taken to prevent liquid from dropping onto the instrument as well as air valve opening and closing position. Carefully monitor the temperature and vaccume pump oil movement. I otherwise scenarios immediately cut off the power supply, contact the concerned person or engineer. Finally, after work open the outlet valve to drain all the water so formed inside the instrument.
Contact Us:
Contact No: 05102730666
Email Id:;
Lyophilizer Charges:
Procedure Name Industry University National LAB/R&D Remarks
  500 per hr 300 per hr    
Additional Rs 100/-towards postal charges if result are to be posted
The Demand Draft should be in favour of ""
Letter, DD & Samples send to "Letter & Samples send to "The Director, IGFRI, Jhansi-284003""
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