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ICAR-Indian Grassland and Fodder Research InstituteICAR-Indian Grassland and Fodder Research Institute
(Indian Council of Agricultural Research)
Near Pahuj Dam, Gwalior Road, Jhansi - 284 003 (UP) India
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  0510-2730666, 2730158
0510-2730385
  FAX: 0510-2730833
igfri_jhansi@yahoo.co.in
igfri.director@gmail.com
 
     
  Seed Tech Developed 2  
 
 

USE OF X-RAY RADIOGRAPHY FOR SEED FILLING IN GRASSES
Range grasses are marked with low seed setting. The actual number of seeds possessing caryopsis is important in studying their seed quality parameters and for further seed research. Manual caryopsis separation is a tedious and highly labour consuming work. A new technique called X-ray radiography was used to study the seed filling in grasses. This technique was proved to be non-destructive and quick method. The data recorded with this method was also verified using manual dehusking and germination test.

Grasses
Percent filled seeds
Grasses
Percent filled seeds
X-ray radiography result
Manual Dehusking Result
X-ray radiography result
Manual Dehusking Result
Bothriochloa intermedia
14.0
12.7
Panicum maximum-BG-1
26.7
24.7
Brachiaria decumbens
8.0
7.0
Panicum maximum-BG-2 -Dc
44.0
44.3
Cenchrus ciliaris-CAZRI-75
31.7
32.3
Panicum maximum-BG-2 - Hvt
27.3
26.3
Cenchrus ciliaris - CAZRI -2178
44.3
45.7
Panicum maximum-wild hairy
33.0
30.3
Cenchrus setigerus
20.7
17.0
Pennisetum pedicellatum-BD-1
28.0
26.0
Chrysopogon fulvus
18.0
14.7
Pennisetum pedicellatum-BD-2
22.0
20.3
Dicanthium annulatum
23.7
21.3
Sehima nervosum
17.3
14.3
Heteropogon contortus
45.3
44.3
Setaria anceps
6.0
4.7
Panicum maximum-Hamil
32.7
34.3
Setaria sphacelata
9.0
7.3

*species specific preconditioning like soaking in hot water (Cenchrus ciliaris, Berseem, Lucerne), longitudinal sectioning of ¾ of endosperm (Paspalum notatum), nipping of seed coat of imbibed seeds (Desmanthus virgatus, Indigofera astragalina and Stylosanthes seabrana) and by chipping of seeds (Clitoria ternatea) is needed for accurate Tz staining.

 
 
 
 
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Seed Tech Developed 1
Seed Tech Developed 2
Seed Tech Developed 3
Seed Tech Developed 4
Seed Tech Developed 5
Seed Tech Developed 6
Seed Tech Developed 7
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